Background: Pincer nail is the transverse overcurvature of the nail plate. Both conservative and surgical treatments can be used to treat pincer nail. However, there are no reports regarding the non-surgical, laser treatment of pincer nail. Objectives: To evaluate the efficacy and adverse effects of combination of CO₂ fractional laser and topical agent on pincer nail. Methods: Eleven pincer nails (All great toenails) were treated with CO₂ fractional laser. An antifungal agent (in case of co-existing onychomycosis) or 20% urea cream were applied to the toenails. We evaluated the difference of the width index, height index, nail angle, and the level of pain before and after treatment. All adverse events and recurrence were observed until the last follow-up visit. Results: The mean number of laser treatments was 2.27. The mean follow-up period after the last treatment was 18.36 weeks. The width index, height index, and nail angle was significantly changed after the treatment. The width index increased by 0.16 (p-value 0.0016), the height index decreased by 1.89 (p-value 0.008), and the nail angle increased by 29.60° (p-value 0.005). The level of pain was reduced in all cases. There were no serious adverse events. Conclusion: CO₂ fractional laser and topical agent combination therapy can be an effective and tolerable treatment for pincer nail. To confirm this result, larger studies with long-term observation are needed.