Background: Saline groundwater collected from the east coast of Korea demonstrated protective effects against 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD)-like skin lesions in murine model. Objectives: To determine the effect of saline groundwater solution baths as the treatment of mild-to moderate AD Methods: 24 subjects with mild to moderate AD were instructed to take a bath with saline groundwater solution for 20 minutes a day for two weeks. Evaluations were performed at baseline and week 2, including SCORing Atopic Dermatitis (SCORAD) index score, corneometry, trans-epidermal water loss (TEWL), visual analog scale (VAS) for pruritus and collection of adverse events. Results: Subjects showed significant improvement with respect to SCORAD index score, skin hydration, TEWL, and pruritus at weeks 2 when compared with baseline. Conclusion: Saline groundwater solution baths might be used as an alternative therapeutic strategy for the treatment of AD.