Recent emerging evidences suggested that myocyte enhance factor 2 (MEF2) and peroxisome proliferator-activated receptorγ coactivator 1α (PGC-1α) play a pivotal role in GLUT4 expression. Present study investigated regulatory effects of exercise training on protein expression of MEF2, PGC-1α and GLUT4 in insulin resistance obese Zucker rats. Investigation was further extended in two different muscle fiber types(soleus, SOL; type I vs. extensor digitorum logus, EDL; type II). After 8 weeks of treadmill running procedure, oral glucose tolerance test (OGTT; 2g/kg body mass; BM) were underwent in all groups(lean Zucker control rats; LN, obese Zucker control rats; OB, exercise-trained obese Zucker rats; EXE) and blood glucose, insulin, and lipids were measured. Exercise training significantly improved insulin sensitivity and blood lipid profiles. There was no significant differences in protein expression of MEF2C, PGC-1α and GLUT4 between OB and LN, except MEF2C in EDL. However, endurance exercise training significantly elevated protein expression of MEF2C, PGC-1α and GLUT4 from OB or LN in both muscle fiber types. These findings indicate that adaptation of skeletal muscles to exercise training increased in protein expression of MEF2C, PGC-1α and GLUT4 in obese Zucker rats skeletal muscles.