In this longitudinal study, measurement of osteo sono index (OSI) of the calcaneus were used as indications of bone quality in community-dwelling elderly. To investigate the possibility of using number of steps walked as an indicator of the role played by physical activity on bone quality, we studied the relationship between OSI and the daily ambulation activity. The subjects were 173 community-dwelling elderly aged 65 or above. Daily walking steps were recorded using a pedometer attached the accelerometer for an year outcome measure of physical activity. The daily steps per day of normal and osteopenia group significantly higher (p<0.05) than osteoporotic group. In elderly male, the subjects who have weight-bearing exercise habits had significantly higher (p<0.05) OSI level. In contrast, weight-bearing exercise habits is no relationship with OSI in elderly women. However, there were higher positive relationship (r=0.51, p<0.05) in women. The daily steps per day may be suitable for evaluating the relationship between ultrasonic bone parameter and physical activity in elderly. Not only the daily ambulation activity, but the presence of exercise habit at present and strength of exercise might influence OSI in male. In contrast, the daily ambulation activity of over 7,500 step is helpful to prevent age-related bone loss in elderly women.