The purpose of this study was to analyze the relationship between ACE genotype and the physical fitness of soccer players. It was conducted a survey on 98 soccer players. Subjects were classified in accordance with ACE genotypes. Statistic method was One-way ANOVA. First, frequency of ACE genotype were ID genotype > II genotype > DD genotype. Second, muscular endurance and cardiorespiratory endurance of II genotype had a tendency that generally had more levels than ID and DD genotype. There was no differences about physical fitness based on ACE genotype as well. Also there was no relationship between different genotypes. It included both aerobic and anaerobic in its speciality and they did training under the same conditions, and did play the game without any condition. In order to improve the player's individual ability, different program should provided to subjects based on their genotypes. Finally, to get a more accurate results about the relationship among those, researchers proposed future study based on different sports types and level of players.