This study was performed to investigate that the effect of walking types on energy expenditure, oxygen consumption (VO₂), heart rate (HR), respiratory exchange rate (RER), the rate of perceived exertion (RPE) and walking frequency during submaximal exercise test (@HRmax 60%). Two different walking types either forward walking (FW) or backward walking (BW) were used in this study. Ten male University students were recruited. Energy expenditure was significantly different between BW and FW (p＜.001), and it was also significantly different between each sections: 3㎞, 4㎞, 5㎞ and 6㎞. VO₂ also revealed a significant different between BW and FW (p＜.01), and there was a significant different between each sections: 4㎞, 5㎞, and 6㎞. Heart rate was significantly different between BW and FW (p＜.01), and it was also significantly different between each sections: 2㎞, 3㎞, 4㎞, 5㎞, and 6㎞ However, RER did not show any statistical significance between walking types when repeated measurement was used. In addition, there was an only significant difference at 6㎞ section RPE scale also revealed a significant different between BW and FW (p＜.001). When the comparisons made by each sections, there was a significant different at 4㎞, 5㎞, and 6㎞ The results from walking frequency, it was a significant different between BW and FW (p＜.01), and there was a significant different between each sections: 2㎞, 3㎞, 4㎞, 5㎞, and 6㎞. While previous studies used only specific walking speed, various walking speeds were used in this study, therefore it was possible to demonstrate that the differences occurred by the change of walking speeds. From these results, it is expected that more effective exercise program can be developed for people who want to maintain and improve their general health conditions, and it could also provide useful information for the determination of exercise intensity which can possibly be used for scientific and systematical guideline for exercise prescription.