The purpopse of this study was to determine the effect of pyridoxine supplementation on ventilation(VE), maximal oxygen uptake(O₂max), respiratory quotient(RQ), O₂pulse, maximal heart rate(HRmax), rating perceived exertion(RPE), and TC/HDL-C ratio. 14 college females were participated in 30 days study and they were randomly assigned to one of the three groups, i.e., GroupⅠ(n=4): placebo (2.4mg/day); GroupⅡ(n=5): pyridoxine (1.6mg/day); GroupⅢ(n=5): pyridoxine(2.4mg/day). Study subjects completed a maximal oxygen uptake treadmill test(modified Bruce protocol), and measured a self reported rating perceived exertion, before and after the treatment. The scheffe's multiple range test, analysis of covariance, and analysis of regression were used to determine the statistical significance. There were no group differences in the VE, O₂max, RQ, HRmax, RPE, exercise works, and exercise time, and TC/HDL-C ratio, but O₂pulse was significantly changed(p〈0.05). In conclusion, it appears that taking 1.6-2.4 mg/day for 30 days of pyridoxine cannot increase the aerobic exercise capacity and TC/HDL-C ratio in college females.