Poly (ε-caprolactone) has been employed in various in vivo medical devices. This FDA approved polymer have been combined with other polymers or methods to improve hydrophilicity, biocompatibility, cell viability, osteo differentiation inducing ability and many other properties for osteogenic regeneration and eventually electrospinning to take advantages of nonwoven structure. With bone xenograft material, osteogenic properties of PCL nonwoven scaffold are expected to be boosted significantly. Additionally solvent vapor post processing was done, which is expected to change fiber morphology and overall structure, resulting in. In this study, we fabricated electrospun scaffold with high concentrated bone xenograft material horse bone powder (HBP), which was post-processed by Dichloromethane vapor (HHBP-NS/SP). Pre-osteogenic cells were cultured to observe effects of post processing on HHBP-NS/SP.