Klitgaard(1988)가 개발한 웨이트트레이닝 모델을 이용한 운동부하가 골격근의 단백합성능과 근섬유의 형태에 미치는 영향을 검증할 목적으로 Sprague-Dawley계의 숫컷 흰쥐 30마리를 대조군과 실험군으로 나누어 실험군은 3개월간 운동을 부하하였다. 1개월마다 체중과 근의 무게를 측정하고 전경골근을 적출하여 표본을 제작, 은 콜로이드 염색과 SDH염색을 하여 유침하에서 광학현미경으로 근핵내의 Ag-NOR수와 근섬유를 계산하여 다음과 같은 결론을 얻었다. 1. 실험군적 체중은 대조군보다 빠르게 증가한 반면 전경골근의 무게는 느리게 증가하였다. 2. 운동부하후 전경골근은 3개월째에 Ag-NOR수가 유의성 있게 증가하였다. 3. 운동부하기간이 길수록 근핵내에 4개이상의 Ag-NOR을 함유한 핵의 비율은 증가하였다. 4. 근섬유의 형태는 운동부하 기간이 길수록 A형(적색 근섬유)는 감소하고 C형(백색 근섬유)는 증가하였다.

This study was to investigated the nucleolar organizer region(NOR) associated protein by means of silver chloride staining and muscle fiber types in sections stained for SDH in the tibialis anterior muscle loaded with movement by klitgaard`s weight training model. Ag-NOR technique is a indirect measure of the ongoing transcriptional activity of the rRNA gene or their activity. The number of Ag-NORs sites within nucleus may reflect protein synthetic activity and proliferation. Thirty spraque-Dawley adult female rat(190-200g) were divided into control and 3 experimental groups were trained for 1.2.3 months. Body weight and muscle weight measured at 1. 2. 3 month. The removed muscle fixed by immersion, and processed for light microscopy. The result were as follow; 1. Body weight were slowly increased, in experimental group, but muscle weight were step by step increased with the lapse of time of training 2. After movement, The mean number of nucleolar organizer regions per nucleus were significantly increased at 3 month in tibialis anterior muscle. 3. The percent of nucleus include 4 Ag-NOR or more was increased with the lapse of time of training. 4. In muscle fiber types, type A were decreased and type C were significantly increased with the lapse of time of training