Purpose: The purpose of this study was to examine differences in awareness and practice of oral health care in pregnant women with and without oral health care education experience. Methods: Participants in the survey for this study were 221 pregnant women in female exclusive hospitals located in B city and G city. Data were collected from October to December, 2014 using self-report structured questionnaires and analyzed using SPSS/Win 21.0 program. Results: The number of pregnant women experienced in oral health education was 36.2%. The oral health care awareness of pregnant women experienced in oral health education total score was 15.14±1.96, and inexperienced pregnant women total score was 14.14±2.63 (t=3.26, p=.001). The oral health care practice of pregnant women experienced in oral health education total score was 12.90±2.48, and inexperienced pregnant women total score was 10.95±4.06 (t=3.89, p<.001). The relationship between the awareness and practice of oral health care was positively correlated. Conclusion: Based on this study outcomes, considering the importance for oral health education in the antenatal care program when the pregnant women visiting the hospital for medical check-up.