We studied removing heavy metal using Bacillus subtilis spore surface display system. We used cot E protein as an anchoring motif because of its high abundance in coat layer and inserted varying length of histidine tag at the C-terminal end of anchoring motif. We checked the surface expression of histidine tag using flow cytometry with FITC labelled anti-his6-antibody. Histogram of flow cytometry showed higher fluorescence intensity, possibly due to their outer location on Bacillus subtilis spore. We tried heavy metal adsorption with three kind of recombinant spore and DB104(wild type).