목적: The goal of this study is to determine the effect of sulforaphene with cisplatin combination treatment on ovarian cancer cell and to reveal potential mechanisms responsible for the effect. 방법: OVCAR-3 cells were treated with sulforaphene and cisplatin. After treatment, preliminary screening was done through 3-(4, 5-dimethylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) Assay. Reactive oxygen species (ROS) and mitochondrial membrane depolarization were studied. Furthermore, to explore the signaling mechanism of sulforaphene, western blot was conducted. Additionally, the expression of caspases-3, 8, 9, phosphoinositide 3-kinase (PI3K), and phosphatase and tensin homolog (PTEN) was analyzed. 결과: Sulforaphene enhances cisplatin efficacy through simultaneous activation of mitochondrial and external pathway of apoptosis as well as modulation of the expression of PI3K and PTEN. 결론: Combination treatment with the sulforaphene and cisplatin resulted in a tic increase in cytotoxic potency and apoptosis in human ovarian cancer NIH: OVCAR-3 cells. Our results suggest that sulforaphene as a chemo-enhancing adjuvant could improve the efficacy of cisplatin in treating ovarian cancer.