목적: Finding a better, safer source of transfusion for acute and chronic blood loss in obstetrics and gynecology is a vital aspect of patient care. With the recent increase in blood-borne diseases and decrease in the candidates for blood donation, finding an autologous source of transfusion is important. Here we propose a method in which discarded human endometrium is used to derive induced pluripotent stem cells and erythroblasts are differentiated as a source of transfusion. 방법: Human endometrial iPS (heiPS) cells were driven from women in their 5th decade receiving hysterectomy. After passaging the heiPS beyond 5-6 passages, the cells were co-cultured with OP9. Cells with radial expansion were sorted with flow cytometry analysis with CD43+, CD34+, CD235a+, KDR+ and CD15+. From day 0-7, the cells were cultured in StemPro-34 SFM medium supplemented with hydrocortisone, stem cell factor and interleukion-3. From day 8-14, cells were cultured in same medium with SCF, IL-3 and recombinant EPO. From day 15-18, the medium was replaced with serum-free conditioned medium with SCF, EPO and poloxamer 188. No cytokines were added and only the poloxamer 188 was used during day 19-21 of culture for enucleation. Giemsa staining was performed on day 7, 10, 14 and 17 to check for morphology and differential count. 결과: Efficient generation of erythroblast from heiPSC was possible using an OP9 coculture system to induce hematopoietic differentiation followed by selective expansion of erythroid cells in serum-free media with erythropoiesis-supporting cytokines. After co-culture on OP9, 12%~13% of CD43+, 3% of CD34+ hematopoietic cells were observed. Analysis of the erythroid cultures by morphologic analysis Wright-Giemsa stained cytospins were revealed that after 7-17 days of expansion, all hiPSCs showed terminal maturation into polychromatic and orthochromatic erythroblasts. 결론: Overall, these results demonstrate the feasibility of the production of erythroid cells from endometrium driven iPSCs.