Objective: Vaginal hysterectomy technique has a common complication such as bladder perforation. We aimed to quantify the distance of the dissection plane from the cervico-vaginal junction to the anterior peritoneal reflection for vaginal hysterectomy in Korean population. Methods: We retrospectively analyzed patients who were undergone vaginal hysterectomy operation at Konkuk University Medical Center from 2016 to 2018. The distance from the cervicovaginal junction to the anterior peritoneal reflection was measured by transvaginal ultrasonogram preoperatively. The distance from anterior colpotomy incision to anterior peritoneal reflection was measured immediately after hysterectomy in the operation room. Results: Total 43 patients were included in this study. The median age was 51.0 (36-83) years and the median BMI was 24.2 (13.0-63.1). Premenopausal women was 55.8% (n=24), over 3 parity was 27.9% (n=12). The most common surgical indications was myoma uteri (34.8%). The median distance from the cervicovaginal junction to the anterior peritoneal reflection measured by transvaginal ultrasonogram was 19.7 mm (7.2-37.1) and the median distance from anterior colpotomy incision to anterior peritoneal reflection measured intraoperatively was 33.0 mm (15.0-55.0). Conclusion: When performing a vaginal hysterectomy, surgeons can expect a median dissection distance from cervico-vaginal junction to anterior peritoneal reflection around of 33 mm which can help to decrease complication of bladder perforation.