The purpose of the study was to examine the effects of regularly forced treadmill running on spatial memory and EEG change in rats performing in a multi water maze. In the study, twenty SD rats were assigned to either a control group(C) or an exercise group(E). Group E performed regularly forced treadmill running for 6 weeks, the escape time and EEG were measured during the water maze test. The activation of EEG was measured in Theta, Alpha, SMR, Beta, and Gamma waves, which were analyzed using one-way ANOVA. In conclusion, there were two major findings from the present study. First, there was a significant reduction in escape time in group E, whereas group C did not show any significant changes, which indicates that regularly forced exercise plays a positive role in enhancing spatial memory in rats. Second, Theta and SMR waves in the frontal cortex were significantly activated in group E. These present results would contribute to the current efforts in developing neuro-feedback programs for the improvement of motor performance.