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고분해능 질량분석기를 이용한 폐사조류 중 다성분 잔류농약 탐색
Residual Multi Pesticides Screening of Dead Birds by High Resolution Mass Spectrometry
이두희 ( Doo-hee Lee ) , 허유정 ( Yujeong Huh ) , 김보경 ( Bo-kyong Kim ) , 정현미 ( Hyen-mi Jung ) , 최종우 ( Joowoo Choi )
UCI I410-ECN-0102-2018-500-003844742

The objective of this study was to evaluate screening method of residual multi pesticides in dead birds by Orbitrap high resolution mass spectrometry (HRMS) to identify the cause of death for birds. Extraction and clean-up method of residual pesticides in stomach and liver of dead birds was used QuEChERS (Quick Easy Cheap Effective Rugged and Safe) EMR method. Method validations were evaluated by linearity, method detection limit (MDL), limits of quantitation (LOQ), accuracy and precision using liquid chromatography and gas chroamtography with triple-quadrupole mass spectrometer (LC/MS/MS and GC/MS/MS). Also, we were evaluated screening method for the determination of residual pesticides in stomach and liver of dead birds by LC and GC Orbitrap high resolution mass spectrometry. Detection and identification were determinated on mass accuracy, isotope pattern, MS/MS fragment pattern and library match by deconvolution program. Results of method validations, Correlation coefficients of the matrix matched calibration curves were >0.978, and the method detection limits (MDLs) and limits of quantitation (LOQ) were 2.8~72.1 ng/g (18.4 ng/g on average) and 9.0~230 ng/g (58.5 ng/g on average). The accuracy ranged from 69.1% to 130% (103% on average), and the precision values were less than 14.8% (3.8% on average). The screening of residual pesticides in stomach and liver of dead birds by LC and GC Orbitrap HRMS was detected monocrotophos, carbofuran, carbosulfan, deltametrin, benfuracarb, carbofuran, phosphamidon and prochloraz in investigated samples. This results showed that accurate mass were extraction of residual pesticides in dead birds by Orbitrap HRMS. It suggested that this screening method is applicable to the residual pesticide analysis for the cause of death as a main tool.

[자료제공 : 네이버학술정보]
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