Background: As one of the methods of measuring the accuracy of staining in special staining, there is a method of evaluating the accuracy of staining using positive control. The positive control is carried out in order to see the structure of a tissue and to check the microorganism infected functionally or externally. In AFB staining, it is necessary to evaluate the accuracy of staining by staining a positive control group, together, in order to evaluate that. The existing method used positive tissues into which tubercular bacilli invaded as a positive control, but it became more difficult to supply this, constantly, as the rate of the initial discovery of tuberculosis increased. So, in order to maintain quantitative, qualitative staining, the need for the production of a positive control block using an acid-fast stain was brought forward. Thus, if the control block is produced, integrated with an AFB stain by the process of producing a cell block used in a cellular pathology laboratory, the quantity or distribution of bacteria is constant in the section because of the characteristic of the cell block. Such a block was produced as it was expected that it could serve as an AFB positive control sufficiently. Methods: The types of the culturing medium include Ogawa medium, a solid medium and Middlebrook 7H9, a liquid medium. A control block was produced through the following procedures: First, scoop out the colony of bacteria cultured on the solid medium, Ogawa medium and mix physiological saline for vortex mix. Put egg albumin in it like producing a cell block. Then, spin it in a centrifuge and process it with an automatic tissue processor. On the liquid medium, Middlebrook 7H9, it was produced in the same way. First, spin the cultured bacteria in a centrifuge. Put them in a cassette and process them with an automatic tissue processor. Results: In a control block cutting and a microscopic examination of the AFB stain, it is found that the number of bacteria distributed on the slide was more on the liquid medium than on the solid medium, which is suitable as a control block. Conclusion: 1) Producing an AFB Control Block with the process of producing a cell block has a merit that it is easy to produce and supply it at any time; 2) There were more bacteria and they were homogeneous when the AFB control block was made by culturing on the liquid medium than on the solid medium, so it could maintain a better quality; and 3) It is concluded that using a harmless NTM stain allows the lab staff who sections the control block to produce slides safely.