Preparation of decyl glucoside D-glucose with 1-decanol was studied on various zeolite catalysts by direct glucosidation. The estrogenicity of decyl glucoside was evaluated to confirm the possibility as an alternative surfactant of nonyl phenol. Catalytic properties of the microporous zeolite in the preparation of decyl glucoside from D-glucose with 1-decanol by single-step direct glucosidation. The zeolite catalysts were consisted of different acidic properties and pore topologies. The effect of acidities and pore topologies of the zeolite catalysts were discussed considering to the conversion and yields to the glucosidation. The estrogenicity of decyl glucopyranoside was estimated using E-assay method to compare that of nonylphenol. The high conversions were obtained inversely to the order of acid strength of the zeolite catalysts. The decyl glucopyranoside represented apparently lower estrogenic cell proliferation compared with nonylphenol.