We introduce a novel colloidal glucose sensing platform technology, in which 2-D arrayed conductive elastomer microparticles of which surface is uniformly coated with silver nanowires (AgNWs) are used as transducers. The essence of our approach is to detect conductance changes induced by covalent bonding of glucose onto the AgNWs on the microparticles. For quantitative detection, we positioned AgNWs/PU conductive microparticles in the holes that are patterned in a stencil substrate. Finally, we demonstrated experimentally that the colloidal glucose sensor developed in this study could detect the glucose over a wide concentration range with remarkable sensitivity.