Mixed Oxides with spinel structure have received great attention for decades in the field of electrolytes, refractories, and catalytic material due to their optical, electrical, and catalytic properties. Highly ordered mesoporous AB₂O₄ (A=Mn, Co, Ni, and Zn, B=Mn, Fe, Co) spinel materials have been synthesized through nanocasting method, using KIT-6 silica as a hard template. From low-angle X-ray diffraction (XRD) patterns of highly ordered mesoporous of AB₂O₄ (A=Mn, Co, Ni, and Zn, B=Mn, Fe, Co) spinel materials, a new (110) peak was observed before 2θ=1°. It indicates the meso-structure transformation from cubic to tetragonal or the lower space group after the silica template etching process. N₂ adsorption-desorption isotherms exhibit the obtained replicas have high specific surface areas (92-125 ㎡/g), and large pore volumes (0.11-0.26 ㎤/g).