Thermo-sensitive hydrogel which happen to physical or chemical change in specific temperature can be applied to a medical and cosmetic fields such as sustained release drug delivery, wound dressing, and skin care. Many references about thermos-sensitive hydrogels have studied the reversible sol-gel phase transition at specific temperatures using polymers such as Methoxypoly(ethylene glycol)-Poly (caprolactone)(mPEG-PCL), poly(lactic acid-co-glycolic acid)(PLGAPEG- PLGA), and poly(ethylene oxide-propylene oxide-ethylene oxide) (PEO-PPO-PEO). We have conducted study using poloxamer 407 with PEO-PPO-PEO structure and gelling agent. For in vivo application, the Sol-Gel phase transition time of the thermo-sensitive polymer was determined at body temperature of 37°C. Physicochemical properties and drug release pattern of the hydrogel was evaluated to suit the purpose of the drug delivery device.