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18.97.14.89
18.97.14.89
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Efficient 5-aminolevulinic acid production through elaborate flux redistribution toward glyoxylate shunt in Escherichia coli
예대열 , 노명현 , 임현규 , 박성훈 , 서상우 , 정규열
UCI I410-ECN-0102-2018-500-003812724
This article is 4 pages or less.
* This article is free of use.

Microbial production of 5-aminolevulinic acid (ALA) has received much attention because of its potential in clinical applications. Overexpression of related enzymes and an transporter yielded remarkable achievements in ALA production. Nonetheless, there is significant room for carbon flux optimization to enhance ALA production. The aim of this study was precise carbon flux optimization for high ALA production in Escherichia coli. Initially, genes hemA and hemL were overexpressed with strong promoters and synthetic 5'-untranslated regions (5'-UTRs). Then, TCA cycle was blocked to force carbon flux toward the ALA production pathway by deletion of sucA. In spite of metabolic imbalance in the strain, further precise tuning of carbon flux to the glyoxylate cycle by varying the transcriptional strength of aceA led to substantially improved cell growth and ALA production.

[자료제공 : 네이버학술정보]
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