Purpose: The purpose of this study was to develop a hand hygiene education program that reflects the characteristics of hospitals and nurses in Cambodia. Methods: To do this, hand hygiene program was developed according to the analysis, design, development stage of ADDIE instructional design model. Results: The result of developed education program is as follows. The education subject is ‘Promotion oh hand hygiene performance of Cambodian nurses’. Education targets are nurses of CPA (Complementary Package of Activities) level 3 hospitals in Cambodia. Educational achievement is to improve the perception of the necessity of hand hygiene and competence in the clinical application of hand hygiene related knowledge of Cambodian nurses. The program will be held once a year for 110 mins. Lecture, demonstration, and discussion are selected as teaching methods. Teaching material are PowerPoint, printed materials, and videos. To evaluate the effect of the program, survey on knowledge, perception, and performance will be carried out one month before and after the class. Conclusion: Through the ADDIE instructional design model, ‘Customized hand hygiene education program for Cambodian nurses’ was developed and this program can be applied in other developing countries.