The Doam-dam watershed, located at Kangwon Province, Korea, has been experiencing significant changes in land uses, conversion from forest to agricultural/urban areas, with human involvements. However, no thorough investigation of the landscape impacts of land use changes was performed at this watershed using scientific analytical tool. Thus, the FRAGSTATS model was utilized to quantitatively analyze the landscape impacts of forest fragmentation in this study. To provide the detailed explanations for 11 landscape indices considered in this study, two artificial and simplified landscapes, before and after fragmentations, were constructed. Using these 11 indices, the landscape impacts of forest fragmentation in 19 subwatersheds of the Doam-dam watershed were analyzed. The S1 subwatershed, one of 19 subwatersheds of the Doam-dam watershed, was found to have experienced the significant forest fragmentation from 1985 to 2000 based on landscape analysis. The results obtained in this study can be used to evaluate the water quality impacts of forest fragmentations/landuse changes at watershed scale level, and establish environment-friendly land use planning based on the results obtained using landscape analytical tool, FRAGSTATS.