Highbush blueberry is a shallow-rooted crop that is highly sensitive to soil water deficits. The amount of water supplied to blueberry plants influences vegetative growth, fruit size and quality. Supplemental irrigation is almost always needed for maximal yields even in years of plentiful rainfall because rain events occur irregularly resulting in periods of drought during the growing season. Despite this vulnerability to water, little is known about the water relations of this crop, particularly with regard to any variation that may occur among cultivars or with soil depth. Therefore, this study was conducted to determine the effects of soil depth and blueberry cultivars on the changes of soil water content. Blueberry plants have shallow root systems with the majority of the roots 20 to 30cm deep in the soil and its distribution is dependent on the age of the plant and climatic and soil conditions. A total of 17 blueberry cultivars (Duke, Elizabeth, Brigitta, Camellia, Rebel, Huron, etc.), 10 cultivars from “A” farm (3,516m²) and 7 cultivars from “B” farm (1,893m²), were investigated. Time-dependent soil moisture data from 100 blueberry cultivation pots were continuously monitored using s portable soil water sensor (WT2000) throughout the cultivation period at two depths, 10cm and 20cm, respectively. The analysis of variance (ANOVA) showed that the soil water content for each blueberry cultivar at 10cm were significantly different while no variation of blueberry cultivar was existed at a depth of 25cm. The comparison of blueberry cultivars showed that there was no critical difference existed among them. Continuous study will be conducted to utilize this result to establish the optimal growth condition for blueberry.