Medium-density fiberboard (MDF) is mainly bonded with urea-formaldehyde (UF) resins which emit formaldehyde and difficult to recycle. Hence, this study attempted to produce MDF panel using oxidized starch (OS) as an alternative adhesive and evaluate its recyclability. The OS was prepared through hydrogen peroxide oxidation. Different levels of polyvinyl alcohol (PVA) were added during the oxidation to improve its adhesion performance. Blocked pMDI (B-pMDI) was added at 1% level based on the OS solids content as a crosslinker. Fourier-transform infrared (FTIR) spectroscopy showed that the oxidation reaction altered the -OH of starch into C=O of carbonyl and carboxyl groups. In addition, FTIR spectroscopy detected that ester group was formed through ether linkage between free -NCO of B-pMDI and the carboxyl group of OS. For recycling analysis, the panels were cut into small cubes. The MDF cubes were hydrolyzed in water at 25 and 80°C for 6 and 1 h, respectively. The mass loss of MDF bonded with OS-PVA was higher than those of UF resins in both hydrolysis conditions. The mass loss of MDF was reduced as the PVA level increased; indicating the addition of PVA improves the adhesion in MDF. Moreover, higher mass loss was obtained after hydrolysis at 80°C for 1h than that of 25°C for 6h. The pH of extract solutions revealed that lower pH was obtained after hydrolysis at 80°C for 1h than that of 25°C for 6h; showing that more OS-PVA adhesives were dissolved after hydrolysis at 80°C for 1h. Higher fiber disintegration was also obtained after hydrolysis at 80°C for 1h, and the values decreased as the PVA level increased. This study suggested that OS-PVA could be used as an adhesive to manufaacture MDF with high recyclability compared to the MDF bonded with UF resins.