Water stress measurements of rice that use spectral reflection characteristics have been conducted to detect a water stress of rice during growing stage. Water stress reaction was measured using a spectroradiometer in the wavelength range from 300nm to 1100nm. In order to maintain all the experimental conditions, the measurement time have been settled at a fixed solar radiation times. Spectral reflectance were measured as a function of the moisture content. The results indicate that the spectral reflectance of rice was dependent on moisture content. As the moisture content is lowered the spectral reflectance of rice formed in NIR wavelength range increased. This effect can be best explained in terms of water stress.