Oxalis corniculata L. (Oxalidaceae) has been used as a natural remedy to help relieve hemorrhoids, boils and dermatopathies. This study was conducted to investigate the anti-aging, anti-oxidation activities and cell toxicity of water and ethanol extracts obtained from O. corniculata by ultrasonic extractor at 40℃, to characterize their practical utility. The content of total polyphenol compounds was higher UW (33.95 mg/g) than UE (18.61 mg/g), total flavonoid compounds was roughly twice as much UE (20.28 mg/g) than UW (10.26 mg/g). The tyrosinase inhibitory, collagenase inhibitory, ABTS and DPPH radical scavenging activities of the ultrasonic ethanol extract, UE were over 1.2 times higher than that of the UW. The xanthine oxidase inhibitory rate of UE (32.71%) was higher than UW (26.41%), in 1.0 mg/mL. The elastase inhibitory activity of UE and UW were 41.75% and 48.00%, respectively. Nitrite scavenging activity was the highest as 51.05% at UW at pH 1.2 at a concentration of 1.0 mg/mL. Cell viability, as determined using the MTT assay, was significantly increased with increasing concentrations of both UW and UE, and showed no toxicity to RAW264.7 macrophages. The inhibitory effect on NO production in LPS-stimulated RAW264.7 cells was decreased in about 10% of UW and UE. These results suggests that the ultrasonic water and ethanol extracts from O. corniculata have anti-aging and anti-oxidative properties and could be used as a natural source for the development of functional cosmetics.