Hydrologic Simulation Program-Fortran (HSPF) coupled with PEST which is optimization program was calibrated and validated at Bochung watershed by using monitoring data of water quantities and nutrient loading. Although the calibrated data were limited, model parameters of each land use type were optimized and coefficient of determinations were ranged from 0.94 to 0.99 for runoff, from 0.89 to 1.00 for TN loading, and from 0.92 to 1.00 for TP loading. The optimized hydrological parameters indicated that the forested land could retain rainfall within soil layer with high soil layer depth and infiltration rate compared with other land use type. Hydrological characteristics of paddy rice field are low infiltration rate and coefficient of roughness. The calibrated parameters related to nutrient loading indicated generation of nutrient pollution from agricultural area including upland and paddy rice field higher than other land use type resulting from fertilizer application. Overall PEST program is useful tool to calibrate HSPF automatically without consuming time and efforts.