Many restoration projects are underway to revive disturbed streams. In order to achieve successful stream restoration, a variety of microhabitats should be created to promote biological diversity. Research on biological classification of microhabitat is essential for biological diversity. However, research on classification using only physical environmental factors has been carried out. The purpose of this study is to classify and quantify the microhabitat of stream by using macroinvertebrates systematically. In this study, eight wadeable streams and four non-wadeable streams were surveyed to identify benthic macroinvertebrates in various microhabitats. Among the physical environmental factors (current velocity, water depth, substrate), the particle size of the substrate was the most influential factor in the emergence of Habitat Orientaion Groups. Among the HOGs, clinger and burrower were highly correlated with physical environment factors and showed the opposite tendency. Distribution of clinger and burrower according to physical environmental factors showed two trendencies based on current velocity (0.3 m/s) and water depth (0.4 m). In addition, the particle size of the substrate showed three trends (≤-5.0, -5.0 < mean diameter ≤-2.0, >-2.0). Based on the abundance tendency of these two HOGs, the microhabitats were classified into nine types from eupotamic microhabitat to lentic microhabitat. Classification of microhabitat using HOGs can be employ for creating microhabitat to promote biological diversity in future stream restoration plan.