CuAAC(Copper(I)-catalyzed azide-alkyne cycloaddition) click reaction is well known for adaptable reaction that could be carried out under diverse conditions. In this study, we synthesized a new peptidyl compound using intramolecular CuAAC click reaction. CuAAC click reaction cause a transformation of intra 1,4-triazole between alkyne and azide of the peptide resulting in increase of monomer emission and decrease of excimer emission of pyrene. By using this property, we investigated the changes of reaction rate of CuAAC click reaction under a variety of reaction conditions upon solvent systems, wide range of pH, different copper ion sources, with or without catalytic ligands or reducing agents and the position of a azide or alkyne.