Background: Recently, it has been reported that sphingolipid-mimetic compounds have hair growthpromoting effect. These are expected to be applicable to patients with low response to conventional therapies. Objectives: To investigate the hair growth-promoting effect of sphingolipid-mimetic compounds. Methods: A total of 68 patients with pattern hair loss were enrolled in this double blind study and divided into two groups: one group as an experimental group using a newly developed sphingolipid-mimetic compound lotion containing 1% ceramide-HS and the other group as a control group using a simulacrum. Both groups applied each lotion on the scalp once a day for 6 months. We evaluated daily hair loss, hair density, hair thickness, and hair length in an interval of 3 months. Results: Fifty eight out of 68 subjects completed this study. The amount of daily hair loss amount in the experimental group showed a tendency to decrease more than the control group. Hair density and hair thickness revealed a tendency to increase in the experimental group compared to the control group. Hair length was found to be slightly longer in the experimental group than in the control group. There was no serious adverse event in this study. Conclusion: The newly developed sphingolipid-mimetic compound lotion is thought to help reduce daily hair loss, increase hair density and hair thickness, and grow hair length.