Effects of various formulation factors using L_8 orthogonal arrays with the stability of doxorubicin hydrochloride injections(DHIs) were investigated. The degradation of DHI may be occurred by pH, temperature, light and metal ions. It is known that DHI should be stored on refrigerated condition of 4∼8℃ because of its unstability on the room temperature. The employed factors were sodium chloride as isotonic solution, sodium bisulfite or sodium pyrosulfite as an antioxidant, disodium edetate as a chelating agent, methyl parahydroxybenzoate as a dissolution time shortening agent, and hydrochloric acid or citric acid as a pH adjusting agent at 22℃. From the results of L_8 orthogonal arrays, an optimal formula, including sodium chloride, disodium edetate, sodium bisulfite and hydrochloric acid, was obtained and the shelf-life of the formula was determined as 560 days approximately.