Purpose: Since the large hospitals are one of the most intensive energy users among building types in Korea, it is important to investigate and apply appropriate energy conservation measures. There are many researches on energy conservation measures for HVAC system in hospitals, but only few useful guidelines for envelope design variables were existed. The building envelope is one of the important factors to building energy consumption and patients` comfort. The purpose of this study is to suggest the most influential envelope design variables for each end-use energy demand. Methods: 100 samples were generated by LHS(Latin Hypercube Sampling) method. After energy performance simulation, global sensitivity analysis was performed by the regression method. DesignBuilder, Simlab 2.2 and JEPlus were used in this process. Results: The most influencing variables are SHGC, SHGC and VT for heating, cooling, and lighting, respectively. However, the most influencing variable for total energy demand is WWR(Window to Wall Ratio). The analysis was conducted based on the coefficient of variance results. Implications: The six envelop design variables were ranked according to the end-use energy demand.