This paper aimed to evaluate the test results of acute toxicity on effluents which were exempted from the application of ecotoxicity criteria. A total 316 effluent samples exempt from ecotoxicity regulation control were tested. The ratio of effluent samples which exceeded the ecotoxicity criteria (TU > I) was 23.7 %, a little bit higher than in a previous similar study (22.7 %). on effluent samples which were controlled under ecotoxicity criteria. This means that our ecotoxicity management system is inappropriate and applications of ecotoxicity criteria to all effluents (82 industriy categories) needed to improve our ecotoxicity system. Given this background, the same numeric criteria (TU I or 2) for 82 industry categories were proposed. The ratio of effluent samples which exceeded the ecotoxicity criteria (TU > I) with D. magna was 23.7 %, but V. flscheri displayed 14.6 %. As an acute toxicity organism, D. magna seemed to be more sensitive than V.flscheri. The ratio of samples which exceeded TU I with D. magna during 24 h exposure was 35 % and after 48 h 41 %.