Purpose This study was aimed to investigat the effects of treadmill training with rhythmic auditory stimulation on gait in patients with stroke. Methods Three subjects were evaluated using the 10-meter walking test (10MWT) and functional gait assessment (FGA). The protocol included 17 minutes treadmill training with periods of rhythmic auditory stimulation (RAS) for 2 weeks. Results Following treadmill training with rhythmic auditory, subjects showed decreases in the time taken to complete the 10-meter walking test and a increase in gait velocity and cadence. In addition, all subjects showed improvement in the functional gait assessment score. Conclusion Treadmill training with rhythmic auditory stimulation had utility as a program to improve gait ability in stroke patients.