A convenient high performance liquid chromatographic method was established for the quantitative determination and content uniformity test of aclatonium napadisilate preparation. This method was more simple to make the sample solution for injection, and easy to determine the content in the preparation. Aclatonium napadisilate was chromatographed using a Lichrosorb-NH₂ column (4㎜ × 25 ㎝, and acetonitrile-water mixture (83:17) as an eluent at a flow rate of 1.8 ㎖/min. RI-detector response was linear over a range of 0.5∼2.0% aclatonium napadisilate under above conditions. Reproducibility studies gave relative standard deviation of 1.29%.