Background : Measles virus (MV) belongs to the family Paramyxoviridae of genus Morbillus. In many parts of the world, measles represents a serious public health problem, and remains a major cause of worldwide morbidity and mortality among children. Measles epidemics are still found in vaccinated populations. Clinical manifestations include pneumonia, diarrhea, and less frequently, acute encephalitis, subacute encephalomyelitis, and mental retardation. Serological detection of IgM and IgG antibodies to MV may be used to determine recent infection, to screen for susceptible individuals, and to monitor vaccine efficacy. Since the early symptoms of measles are similar to other respiratory tract infections, the rapid demonstration of virus specific IgM antibodies is a valuable laboratory tool for diagnosis. In this study, we have evaluated DSL Measles IgM and IgG ELISAs for their ability to identify the MV antibodies among clinically suspected measles cases, and reliability of the assay for routine diagnosis in comparison to a well established commercially available assay. Materials and Methods : DSL IgM assay is based on u-capture technique while the IgG assay is based on specific, purified, and inactivated whole virus antigen (strain Edmonston) coated on to the microtiter strips. Sera of 391 Korean children and young adults suspected of having measles were supplied from NeoDin Medical Institue in Korea. The sera were collected shortly after the appearance of clinical symptoms. Moreover, we analyzed 52 sera from patients with various serological positives: Rubella (10), Toxoplasma (19), Parvovirus B 19 (13), ANA (10); and 120 random serum samples. Each of these samples was tested using the other commercially available ELISA. Results : In summary, among 391 samples from suspected measles, DSL IgM ELISA detected 304 positives, while the commercial ELISA gave identical results. Of the 52 potentially crossreactive sera, none were positive in DSL IgM ELISA, whereas one was classified positive by the commercial assay. Within the randomly selected group: one was positive by DSL IgM ELISA and two were positive in the commercial assay; 79 and 78 were positive by DSL IgG and commercial assay, respectively. Conclusions : These results support the utility and reliability of DSL Measles ELISA as a screening tool in clinical laboratories.