The effects of mixture of tween 20 and span 20 or span 80 on griseofulvin absorption in rabbit were studied. The plasma concentration of grisevfulvin in blood samples was determined by high pressure liquid chromatographic procedure. Griseofulvin suspension with 0.05% surfactant increased mostly drug absorption and produced about two times higher plasma level of griseofulvin than that of the suspension with 1% and 0.1% surfactant. Surfactants with H.L.B. value 4.3 resulted 2.74㎍/㎖ of maximum plasma levels of griseofulvin at 18 hours after its oral administration and surfactants with H.L.B. value 16.7 resulted 0.56㎍/㎖ of peak plasma levels of griseofulvin at 1 hour after its oral administration.