본 연구는 부생성 균류인 Uloclacdium atrum을 이용하여 Botrytis spp.에 의한 백합 잎마름 병및 오이 잿빛곰팡이병의 방제효과를 조사하고 그 작용특성을 규명함으로서 U. atrum의 국내 최고의 보고이다. 토마토 잎의 표면과 조직 속에서 분리한 Ulocladium sp. CNU 9054균주는 형태적 특징에 의하여 U. atrum의 Botrytis spp.포자형성억제력을 실내검정으로 조사하였던 바,U. atrum은 고사된 잎 절편에서 Botrytis의 포자형성을 효과적으로 억제하였다. Botrytis의 포자형성억제력은 U. atrum을 Botrytis보다 먼저 처리했을 경우 효과가 높았다. 백합 잎마름병 방제를 위한 포장(온실)실험 결과 U. atrum CNU 9037 균주의 포자현탁액 처리는 백합잎마름병을 효과적으로 억제하였으며 방제가는 70%,77%로 procymidone 약제 처리기구의 방제가(58%)보다 우수하였다.U.atrum(CNU 9037,CNU 9054)포자현탁액처리는 오이 잿빛곰팡이병도 효과적으로 억제하였으며, 그 방가제는 66~71%였다. 본 연구의 결과는 U.atrum균주가 오이, 백합의 Botrytis병의 생물학적 방제제로서 개발가능성이 있음을 나타낸다

This study was conducted to investigate the effect of U. atrum treatment on control of Botrytis leaf blight of lily and Botrytis gray mold of cucumber, and to evaluate the U. atrum as the biological control agent of Botrytis diseases. The antagonistic isolates CNU 9037 and CNU 9054 isolated from tomato leaves were identified as Ulocladium atrum Preuss based on morphological characteristics. This is the first record of U. atrum in Korea. In bioassays on dead leaves of tomato and cucumber, treatment of U. atrum colonized the dead leaves and suppressed sporulation of Botrytis as compared with the untreated control. The suppression of spoulation of Botrytis on dead leaf segments by U. atrum was higher when U. atrum was treated before Botrytis was treated. The effect of treatments with conidial suspension of U. atrum on leaf blight of lily and gray mold of cucumber caused by Botrytis elliptica and B. cinerea, respectively, was investigated under greenhouse conditions. Spraying U. atrum (1 × 106 conidia per ml) at intervals of 1 week for three times resulted in a significant reduction of natural infections of lily leaves caused by B. elliptica. Protective value of U. atrum treatment was higher than that of the fungicide (procymidone) treatment. Spraying U. atrum also resulted in a significant reduction of cucumber gray mold caused by B. cinerea. Our results show that U. atrum has a potential for biological control against diseases caused by Botrytis spp. in lily and cucumber.