Flexure toughness of Steel Fiber Reinforced Concrete (SFRC) shows a time-dependent characteristic due to the hydration process of the cement matrix in the SFRC system. The effect of two important factors, water/cement (w/c) ratio and fiber volume fraction, on the flexure toughness development of SFRC were investigated. Three different SFRC mixtures with hooked-end steel fibers were tested using a four-point bending testing configuration. Each mixture was tested at five different ages. The results showed that the post-peak toughness of SFRC developed at an earlier age than the first-crack toughness.