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Pooling 검체에 대한 Antiglobulin Gel Test의 불규칙항체 선별능에 관한 연구
An Antibody Screening Test Based on the Antiglobulin Gel Test, Pooled Test Cells, and Pooled Sera
이정신 ( Jung Sin Lee ) , 한금희 ( Keum Hee Han ) , 최민자 ( Min Ja Choi ) , 김현옥 ( Hyun Ok Kim )
UCI I410-ECN-0102-2016-510-000680931
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The recently introduced gel technique offers significant advances with respect to standardization of test and provides clear and stable reactions that improve the interpretation of results. The purpose of the present study was to design an antibody screening test based on the gel technique using the pooled cells and pooled sera. Four hundred patients` sera were screened for irregular antibodies by the gel technique in parallel with conventional tube indirect antiglobulin test(IAT). Irregular antibodies were detected in 2 sample(anti-Le(a) and anti-Fy(a)) in both IAT and gel technique. Twenty four frozen sera, which the specificity of irregular antibodies was identified at our blood bank, were test for irregular antibodies by antiglobulin gel test using pooled panel cells or pooled serum two sample. The gel technique proved equal to the tube test in the detection of all antibodies except "enzyme-only" anti-E and anti-Le(a). Our main reason for pooling test is financial. We conclude from this study that screening for irregular antibodies using the gel test with pooled panel cells is a sufficiently reliable and sensitive procedure, and saves handling time.

[자료제공 : 네이버학술정보]
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