Objectives: This study was conducted to find the factors associated with performance of blood-borne infection prevention. Methods: The study subjects were 204 operating room nurses from 23 hospitals in G metropolitan city. The data were collected with self-reported questionnaire, and the used statistical methods of analysis were t-test, ANOVA with post-hoc test, Pearson’s correlation analysis and multiple regression analysis. Results: Of the subjects, 41.2% was exposed to the patients’ blood during the surgery. As for the awareness and performance of blood-borne infection prevention, the mean scores of subjects were 107.4±10.8 and 100.6±13.3, respectively. The results of multiple regression analysis suggested that as the awareness of blood-borne infection prevention increased (β=0.6, p<0.001), participation in surgery after the confirmation of infection test results (β=4.1, p=0.042) and hospital’s training for infection control (β=3.4, p=0.040) increased the performance of blood-born infection prevention, whereas experiences in being exposed to blood significantly decreased the performance of blood-borne infection prevention (β=-5.5, p<0.001). Conclusions: It is essential that hospitals prepare aggressive and effective management measures for preventing blood-borne infection and operating room nurses make individual efforts to recognize and perform infection control well despite the limited conditions of the hospitals where they are working.