This study was performed for the purpose of the development of multimedia database for biology instruction of middle school. This database was made of HTML record for each middle unit according to the biology curriculum, and was included a variety of letter, graph, Picture, drawing, animation, and other moving image materials. And it was composed of six coursewares(learning context, inquiry activity, practical science, image material, test item, and terminology dictionary). The coursewares are as follows: 1. Learning context: The main learning context of each middle unit was summarized and explained by the analysis of eight types of science textbooks authorized through the Board of Education. 2. Inquiry activity: This section was contained the explanation for experiment, experimental process, analytic method, and discussion and conclusion. It also offered helpful information to facilitate data analysis and discussion. This section eras included the all inquiry activity sources of eight types of textbooks. That is, there was included 23 types of the lst grade, 36 types of the 2nd grade, and 23 types of the 3rd grade books in this section. 3. Practical science: This section was provided to the form of reading materials for examples of real life, and science history. Special attention was given to the areas of science, technology, and society. This section was included 15 types of the lst grade, 15 types of the 2nd grade, 18 types of the 3rd grade books. 4. Image material: This image material was included calor picture; stop image, and moving image, and 22 types of the 1st grade, 28 types of the 2nd grade, 47 types of the 3rd grade. 5. Test item: This section is the database to test the result of learning activity, and there was included 18 items of the lst grade, 18 items of the 2nd grade, and 57 item of the 3rd grade. 6. Terminology dictionary: A terminology dictionary was provided with the explanation to assist the understanding of student and teacher for difficult terminology concept in the unit. This section was included 124 items of the 1st grade, 147 items of the 2nd grade and 159 items of the 3rd grade. This database is uploaded an Internet website under Science Education Research Institute of Kongju National University, and also is provided to a CD-ROM title. We think that this database may be very useful to practical inquiry instruction because of including a variety of the multimedia instruction materials for the biology field of middle school.