The major purpose of this research was to identify Korean high school students` misconceptions on animal and plant physiology. In order to find out high school students` misconception on animal and plant physiology, the paper and pencil test form of a questionnaire was constructed with 15 items and sent to 541 high school students, Their responses were collected and the data was analyzed. According to the analysis of the questionnaire an animal and plant physiology for students, the general lower academic level students` group had mare statistically significant misconceptions compared to higher level students` group in the areas of animal and plant physiology were concerned. But there was not the statistically significant difference in misconceptions about the concepts of amino acids between the two students` groups. The males had many misconceptions about sperm concepts. In the case of concepts on the excretory organ, especially the students from urban area had less misconceptions than the ones from rural area had.