본 연구에서는 넙치 (평균체장 및 무게: 20±2.0cm, 70±5.0 g)에 옥시테트라사이클린(oxytetracycline, OTC)-네오마이신(neomycin, N) 복합제를 25-10 ppm 및 50-20 ppm으로 용해하여 2일 동안, 3시간씩 약욕을 실시하였고, 약물투여 중지 후 1일, 5일, 14일, 40일째에 시험군별로 각각 5마리씩 넙치의 근육을 채취하여 항생제 잔류농도를 조사하였다. OTC와 N 표준용액을 농도별로 희석하여 표준곡선을 작성한 결과 각각 R2= 0.9999, R2= 0.9952로 양호한 직선성을 나타내었고, OTC와 N 회수율은 각각 90∼93%, 88∼95%가 관찰되었다. 잔류농도에 대한 측정결과, OTC와 N 복합제를 25-10 ppm 투여한 시험군에서 1일째 OTC는 0.97±0.084 μg/ml이 검출 되었고, N은 0.118±0.079 μg/ml이 검출되었으나 5일째 이후부터 검출이 되지 않았다. OTC와 N 복합제를 50-20 ppm 투여한 시험군에서 1일째 OTC는 1.324±0.062 μg/ml이 검출되었고 N은 0.788±0.05 μg/ ml이 검출되었으나 5일째 이후부터 검출이 되지 않았다.

In the present study, we performed a dipping of olive flounder (average length and weight: 20±2.0 cm, 70±5.0 g) for a period of three hours a day, over two days, in a melted complex of oxytetracycline (OTC) and neomycin (N), by dissolving 25-10 ppm or 50-20 ppm in water. Subsequently, the remaining antibiotic density in muscle tissue collected from olive flounder was investigated, 1, 5, 14 and 40 days after discontinuation of the medication. 5 fish were used from each group. The standard graph drawn from the results of diluting two standard solutions of OTC and N based on various density levels, showed a relatively straight line with an R2 of 0.9999 and 0.9952, respectively. The recovery rate of OTC was shown to be 90-93% and N, 88-95%. Upon measurement of the remaining antibiotic density in the test group that had been exposed to 25-10 ppm of the complex of OTC and N, 0.97±0.084 μg/ml of OTC and 0.118±0.079 μg/ml N were detected on 1 day of the test. No antibiotic density was detected after day 5 of the test. Regarding the test group that were exposed to 50-20 ppm of the complex of OTC and N, 1.324±0.062 μg/ml of OTC and 0.788±0.05 μg/ml N were detected on day 1 of the test, and no antibiotic density was detected after day 5 of the test.