Methylene blue method has been mainly used for the analysis of anion surfactants which are an influential pollutant in rivers. But this method have various defects, because its analysis procedure needs long pretreating time. In this study, methylene blue method of anion surfactants was compared with the Co-PADAP method using a bit solvent to simplify the procedures. It was concluded that there is no significant difference between the two methods, so Co-PADAP method must be adapted to the Korean Standard Methods.