Purpose: This study was conducted to identify the level of job stress and to determine factors influencing job stress among nurses currently under Academic Credit Bank (ACB) system. Methods: The participants were 153 nurses who has taken courses of ACB system. Data were collected through self-report questionnaires and then analyzed with t-test, ANOVA, Pearson correlation coefficients, and multiple linear regression with SPSS/WIN 18.0. Results: The score of job stress showed significant differences according to age, marital status, total period of clinical career, and nursing unit. Job stress had significant negative correlation to professional self-concept and organizational commitment. The factors influencing job stress were marital status, professional self-concept, and organizational commitment. Conclusion: The results suggest that various factors should be considered in development and implementation of programs to reduce the job stress of nurses currently under ACB system.