Fractionation and separation of the EtOAc extract from Cimicifuga heracleifolia rhizomes led to the isolation of two major phenylpropanoids, isoferulic acid (1) and isoferulic acid methyl ester (2). The rhizomes of C. heracleifolia were extracted with MeOH by a standard extraction procedure, and the extract was partitioned with n-hexane, EtOAc, n-BuOH and H2O, successively. The EtOAc extract was fractionated and purified using MPLC and HPLC to give two phenolic compounds 1 and 2. Their structure were established based on the UV, MS and NMR spectral analysis.