Scabies is caused by a mite, Sarcoptes scabiei var hominis, an obligate human parasite that burrows tunnels downwards into the epidermis. Scabies may be transmitted from objects, but is most often transmitted by direct skin-to-skin contact, with a higher risk with prolonged contact. Treatment must involve the entire household or community to prevent reinfection. In Korea, the incidence of scabies has decreased dramatically since the late 1980s, but recent outbreaks in nursing homes or hospitals have been raising public health concerns. Herein we describe five cases of scabies occurred in 3 generations of a family, which were confirmed dermoscopically and microscopically. We also used dermoscopy-oriented skin scraping with microscopic examination in evaluating the efficacy of scabies treatment and could treat the collective infestations in a family rapidly and effectively.